[PubMed] [Google Scholar]Sprent J., Hurd M., Schaefer M., Heath W. a crucial part in the attenuation of graft-versus-host disease by regulating the growth of myeloid-derived suppressor cells. gene in pseudoautosomal region 1 (PAR1) of the X chromosome, is definitely a ubiquitous 32 kDa Tolrestat transmembrane protein with a highly O-glycosylated extracellular region (Hahn et al., 1997; Park et al., 2005). It Rabbit Polyclonal to CEACAM21 is expressed in all leukocyte lineages and involved in many cellular events. Engagement of CD99 on human being thymocytes with agonistic antibodies induces homotypic aggregation (Hahn et al., 1997), apoptosis (Bernard et al., 1997), and upregulation of TCR and MHC class molecules on the surface of thymocytes (Choi et al., 1998). In adult T cells, CD99 delivers effective co-stimulatory signals (Oh et al., 2007). In B cells, the downregulation of CD99 by EBV-encoded latent membrane protein-1 (LMP-1) prospects to the generation of Hodgkin and Reed-Sternberg cells related to Hodgkins disease (Kim et al., 1998; 2000; Lee et al., 2011). Moreover, transendothelial migration of monocytes is also regulated by human being CD99 (Schenkel et al., 2002). In addition to these observations in humans, a mouse homologue of human being CD99 (also designated D4) was identified as a ligand of the combined Ig-like type 2 receptor (PILR) (Park et al., 2005; Shiratori et al., 2004). Its practical analogy with human being CD99 is definitely supported by reports that it also participates in the transendothelial migration of leukocytes and recruitment into inflamed cells (Bixel et al., 2004; Dufour et al., 2008). Until recently, however, these studies have been limited to or experiments using CD99-deficient cell lines or anti-CD99 antibody due to the unavailability of CD99-deficient mice. Myeloid-derived suppressor cells (MDSCs) are a heterogeneous populace of triggered immature myeloid cells with morphology much like granulocytes or monocytes (Movahedi et al., 2008) that accumulate under several pathologic conditions including cancer, illness, inflammatory disease, and stress (Gabrilovich and Nagaraj, 2009). MDSCs are characterized by the co-expression of myeloid-cell lineage differentiation antigen Gr-1 and CD11b (Mac pc-1) in mice (Gabrilovich and Nagaraj, 2009). MDSC growth and activation are affected by several factors, representative of which are cyclooxygenase 2 (also known as PTGS2) and vascular endothelial growth factor (VEGF), which are produced by tumor cells, tumor stromal cells, and activated T cells (Gabrilovich and Nagaraj, 2009). These factors are mainly involved Tolrestat in the upregulation of immune suppressive factors in MDSCs and their growth. MDSCs were recently reported to play a potentially important role in determining the severity of graft-versus-host disease (GVHD) (Rao et al., 2003) by suppressing alloreactivity (Highfill et al., 2010; Morecki et al., 2008). In the present study, we found that there was significant aggravation of GVHD when splenocytes of CD99-deficient mice were used as donor cells. In subsequent experiments to identify the mechanism by which CD99 is related to the disease course of GVHD, we found that growth of MDSCs is definitely seriously compromised in the absence of CD99. MATERIALS AND METHODS Mice A heterozygote mutant C56BL/6 mouse clone (CD99+/GT) with an insertional mutation between exons 2 and 3 of the CD99 gene (21-B6T44 clone in Exchangeable Gene Capture Clone database; http://egtc.jp/action/access/clone_detail?id=21-B6T44) was produced using the pU-21T exchangeable gene capture vector and deposited in the Center for Animal Resources and Development (Cards) R-BASE of the Kumamoto University or college of Japan (http://cardb.cc.kumamoto-u.ac.jp/transgenic/index.jsp). Wild-type C57BL/6J (B6, H-2b), B6.C-H2bm12/KhEg (bm12), and CD45.1 congenic B6 mice were purchased from your Jackson Laboratory (USA). CD99GT/GT mice were backcrossed to CD45.1+ congenic B6 mice to generate CD45.1+ CD99GT/GT mice. All mice were managed at the Center for Animal Source and Development at Seoul National University or college. The animal studies were performed after receiving approval from your Institutional Animal Care and Use Committee (IACUC) of Seoul National University or college (IACUC authorization No. SNU-091125-5). Genotyping of CD99GT/GT mice DNA samples for CD99GT/GT genotyping were extracted from Tolrestat your tails of mice and PCR.