Sigma, General – Immune checkpoint inhibitor induces

After purification by ultrafiltration with an Amicon Ultra-15 Centrifugal Filter Unit with a 10 kDa membrane from EMD Millipore (Billerica, MA, USA), the final biotin/antibody ratio was 4 as determined by the HABA method (Pierce)

December 26, 2024 iah2010

After purification by ultrafiltration with an Amicon Ultra-15 Centrifugal Filter Unit with a 10 kDa membrane from EMD Millipore (Billerica, […]

Total modified types of proteins by NEDD8, ubiquitin, and SUMO in cell lysates were analyzed by Traditional western blotting with indicated antibodies

October 25, 2024 iah2010

Total modified types of proteins by NEDD8, ubiquitin, and SUMO in cell lysates were analyzed by Traditional western blotting with […]

High-resolution SNP array was performed using Affymetrix Cytoscan HD following manufacturers protocol

January 3, 2023 iah2010

High-resolution SNP array was performed using Affymetrix Cytoscan HD following manufacturers protocol. WES Handling and Variant Getting in touch with. […]

provided reagent

September 3, 2022 iah2010

provided reagent. Furthermore, expression evaluation of TLR-signaling-related genes in the triggered B2 cell subsets, evaluated using the Toll-Like Receptor Signaling […]

In this examine, we have first dealt with the different modes and pathways involved in cell death and then we have focused on the regulation of several proteins in these signaling cascades by the different deubiquitinating enzymes, in the perspective of cancer

February 13, 2022 iah2010

In this examine, we have first dealt with the different modes and pathways involved in cell death and then we […]

For apoptosis/cell loss of life analysis, cells were harvested, washed once in PBS, resuspended in annexin V binding buffer (Becton, Dickinson, NJ, USA) containing a saturating concentration of an APC-conjugated annexin V antibody and Zombie NIR (BioLegend), and incubated for 15 min at room temperature (RT) in the dark

November 23, 2021 iah2010

For apoptosis/cell loss of life analysis, cells were harvested, washed once in PBS, resuspended in annexin V binding buffer (Becton, […]