For apoptosis/cell loss of life analysis, cells were harvested, washed once in PBS, resuspended in annexin V binding buffer (Becton, Dickinson, NJ, USA) containing a saturating concentration of an APC-conjugated annexin V antibody and Zombie NIR (BioLegend), and incubated for 15 min at room temperature (RT) in the dark
For apoptosis/cell loss of life analysis, cells were harvested, washed once in PBS, resuspended in annexin V binding buffer (Becton, […]