Thereafter, endothelial cells had been isolated simply by collagenase treatment (type We, Worthington, 1 mg/ml for 12 mins at 37C) and cultured in tradition dishes coated with collagen (type We prepared from rat tail; 60 ng/ml) including moderate RPMI1640/M199 (vol/vol) and 15% fetal leg serum supplemented with penicillin (100 U/ml), streptomycin (100 U/ml), fungizon (250 g/ml), L-glutamine (2 mmol/liter) and cultivated for 48C72 hr

iah2010

Thereafter, endothelial cells had been isolated simply by collagenase treatment (type We, Worthington, 1 mg/ml for 12 mins at 37C) […]